Diagnosis of Human Brucellosis by Blood Culture (BACTEC) and PCR Meth- od via Whole Blood and Serum
نویسندگان
چکیده
Background: Brucellosis is the most common global zoonosis and an important public health problem in many parts of the world including Iran. Diagnosis of brucellosis is frequently difficult to establish and conventional methods are not always successful in identifying the organisms. Rapid detection of Brucella species by an automated blood culture system and Polymerase Chain Reaction (PCR) may lead to an earlier diagnosis and may improve patient management. Objectives: The current study aimed to evaluate PCR technique as a diagnostic tool for brucellosis in comparison to conventional bacteriological techniques. Materials and Methods: A total of 50 patients suspected to have brucellosis were included in this study. All patients presented clinical signs compatible with brucellosis. Diagnosis was established by detecting a titer equal to or greater than 1:160 by the standard tube agglutination (STA) method. Blood samples and sera from the patients were tested by culture using BACTEC 9050 system and PCR using primer set to amplify a 223 bp region with in the gene coding for a 31 KD Brucella antigen. Results: Eleven (22%) whole blood samples and 17 (34%) serum samples out of 50 had positive PCR and 7 (14%) patients had Brucella species grown in their cultures. Out of 43 blood culture negative samples, 10 (23.3 %) were positive with the serum PCR versus in 4(9.3 %) with whole blood PCR. Conclusions: The results suggest that the serum PCR assay is rapid and easy to perform and highly sensitive and specific, and it may therefore be considered a useful tool for diagnosis of human brucellosis.
منابع مشابه
تشخیص بروسلوز با استفاده از سیستم کشت خون BACTECو تائید نتایج آن با PCR
Abstract: Brucellosis is one of the most common zoonotic diseases in Iran. Growth of Brucella is slow and blood cultures of these bacteria are time-consuming via classical methods. We try to evaluate BACTEC 9120 system capacity in order to detect of bacteremia due to Brucella spp and to confirm isolated bacteria by PCR. Blood culture sample of 102 suspected patients evaluated by BACTEC 9120 sys...
متن کاملEvaluation of Serum Agglutination Test in the Diagnosis of Pediatric Brucellosis
Background & Aims: Brucellosis is a public health problem in many developing countries, including Iran. Diagnosis is based on history, clinical manifestations and positive serum agglutination test (SAT) or Wright test. The purpose of this study was to evaluate the diagnostic value of SAT in pediatric Brucellosis. Methods: In this prospective study, during 2 years, 52 patients with clinical feat...
متن کاملPrevalence of the Most Common Virulence-Associated Genes among Brucella Melitensis Isolates from Human Blood Cultures in Hamadan Province, West of Iran
Brucellosis is a widespread zoonotic disease causing considerable economic and public health problems. Despite animal vaccination, brucellosis remains endemic in some areas such as Iran, especially in the western Iranian province of Hamadan. We sought to detect some of the most common virulence-associated genes in Brucella isolated from human blood cultures to determine the prevalence of some v...
متن کاملComparison between conventional PCR and PCR - ELISA for detection of Brucella melitensis
Molecular detection techniques are believed to be key tools for both prevention and treatment follow up of brucellosis within live stock and human beings. Consequently rapid, reliable, easy to perform and automated systems for Brucella detection are urgently needed to allow early diagnosis and adequate antibiotic therapy in time. Brucellosis is a worldwide re-emerging zoonosis causing high econ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2013